Production of Chimeric Loach by Cell Transplantation from Genetically Pigmented to Orange Embryos

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Production of chimeric loach by cell transplantation from genetically pigmented to orange embryos.

To establish techniques for chimera formation and to obtain further knowledge of chimerism, chimeric loach were produced using the wild strain as the donor and the orange strain as the recipient by cell transplantation. Transplantation between embryos at two different stages was performed to achieve efficient chimera formation. In the combination of the early-mid-blastula as the donor and the l...

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One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an organism that contains cells from more than one animal; mosaics are one type of chimera in which cells from more than one genotype are mixed, usually wild-type and mutant. In the zebrafish, chimeras can be readily made by transplantation of cel...

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ژورنال

عنوان ژورنال: Zoological Science

سال: 2003

ISSN: 0289-0003

DOI: 10.2108/zsj.20.333